pGLO Observations , Data Recording & Analysis
1.
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Obtain your team plates. Observe your set of “+pGLO” plates under room light and with UV light. Record numbers of colonies and color of colonies. Fill in the table below.
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2.
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What two new traits do your transformed bacteria have?
The first trait that I observed was that the transformed bacteria started to glow, The second trait that I saw that the transformed bacteria were not only growing, but the bacteria were both green and glowing.
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3.
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Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
I think that there are too many bacteria to count in the 100 uL of bacteria. Bacteria are microscopic and with that, it is hard to tell how many single bacterium there are.
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4.
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What is the role of arabinose in the plates?
The role of arabinose is to provide a way to control expression of the GFP gene.
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5.
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List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
The first application of GFP is US Company Yorktown Technologies sold florescent green zebra fish in order to detect pollution in waterways. The second application of GFP is that a Japanese-American team created florescent green cats as a proof of concept to use cats as a model organism for disease. The third application of GFP is that a South Korean Team bred red glowing dogs which allowed scientists to study the genes that cause humans diseases such as blindness.
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6.
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Give an example of another application of genetic engineering.
Genetic engineering can be used to design various things. An example of genetic engineering would be to mass produce human growth hormones in the field of medicine. Genetic engineering can mass produce other various things such as vaccines and insulin.
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